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Cedars-Sinai Postdoctoral Scientist - Department of Medicine - Melmed Lab in Los Angeles, California

The Melmed Laboratory focuses on elucidating mechanisms of pituitary tumor development and pathogenesis, translating the study of anterior pituitary function regulation to the clinic and establishing new modalities of diagnosis and treatment for pituitary diseases. Examples of work in the Melmed Lab include acromegaly therapeutic targeting via somatostatin receptor signaling, Cushing disease drug discovery using a zebrafish model for screening of small molecules, and characterization of the human pituitary tumor-transforming gene, critical to pituitary tumor cell cycle outgrowth.

Job summary:

Working independently but in close cooperation and in consultation with the Principal Investigator and other Research Scientist, as a Postdoctoral Research Fellow, you will perform routine and complex laboratory procedures throughout training period. You may develop, adapt, and implement new research techniques and protocols. You will analyze and interpret data and may assist in preparation of grant proposals. Participates in publications and presentations as author or co-author. Not responsible for generating grant funds.   

Essential job duties and responsibilities:

  • May assist in preparation of grant proposals, but is not responsible for generating grant funds.

  • May participate in publications and presentations as author or co-author.

  • Designs and performs experiments. Will keep appropriate experimental records and documentation and analyze the results with the Principal Investigator.

  • May develop, adapt, and implement new research techniques and protocols.

  • Analyzes, interprets, summarizes and compiles data.

  • Performs routine and complex laboratory procedures throughout training period.

  • Operates and maintains equipment and instruments.

  • Will investigate the mechanisms of pituitary tumorigenesis

Specific laboratory procedures conducted:

  • Plasmid construction

  • DNA/RNA/protein extraction

  • SDS-polyacrylamide gel electrophoresis

  • western blotting

  • immunoprecipitation

  • luciferase reporter gene assay

  • site-directed mutagenesis

  • Glutathione S-transferase (GST) pull-down experiments


  • quantitative real-time PCR


  • IgG purification

  • DNA digestion

  • DNA amplification

  • ligation and subcloning

  • DNA or siRNA transfection

  • direct sequencing

  • mammalian cell culture and transfection

  • blood and organ collection (from rats and mice)

  • isolation of primary hepatocyte (rat)

  • primary culture of anterior pituitary cells (mice)

  • injection into tail vein of mice

  • in vivo knockdown experiment by antisense oligonucleotide

Educational Requirements:

  • Doctorate (MD, PhD, VMD, or DDS) in area directly related to field of research specialization is required


  • Works independently on research projects designed by a mentor (typically the PI) within area of specialization.

  • Acquires thorough technical and theoretical knowledge of research project and objectives during one to five (1-5) year post-doctoral appointment.

  • Demonstrated aptitude to perform experimental protocols and procedures, including detailed data collection, and analysis and operation and maintenance of specialized equipment.

  • Knowledge of safety standards and maintenance of specialized equipment.

Cedars-Sinai is an EEO employer. Cedars-Sinai does not unlawfully discriminate on the basis of the race, religion, color, national origin, citizenship, ancestry, physical or mental disability, legally protected medical condition (cancer-related or genetic characteristics or any genetic information), marital status, sex, gender, sexual orientation, gender identity, gender expression, pregnancy, age (40 or older), military and/or veteran status or any other basis protected by federal or state law.