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Cedars-Sinai Research Associate II - Kumar Lab - iPSC in Los Angeles, California

Summary of Essential Job Duties:

The Kumar Laboratory at Cedars-Sinai Medical Center, Regenerative Medicine Institute is looking for a passionate, inquisitive, and enthusiastic individual who wishes to investigate the cellular and molecular underpinnings of the regenerative responses post -mammalian acute kidney injury with a goal to identify novel therapeutic targets and strategies to augment intrinsic regenerative responses and inhibit fibrosis post-injury. Such approaches will “kick-start” the acutely injured kidneys and prevent its progression to chronic kidney disease. Coupling state-of-the-art transgenic mice to the repair/regeneration model of acute kidney injury the incumbent will study the key biological processes involved in the regeneration of an injured epithelia per se. To gain further insights into such regenerative responses, such responses are also interrogated during kidney development. In addition, the laboratory will leverage on the world-class human induced pluripotent stem cell (iPS) core facilities of RMI to establish systems for kidney disease modeling and investigation of human renal repair responses.

The incumbent will assigned to a funded project investigating the above processes, and will gain critical insights into kidney stem/progenitor cells and kidney repair/regeneration. In order to conduct this research the incumbent should have a primary interest in regenerative medicine and stem cells, molecular cloning, next generation sequencing such as single-cell sequencing, RNA-seq and Chip-Seq, high resolution imaging, and Crispr/Cas9-based gene editing methodologies. The ideal candidate will have outstanding molecular and cell biology training with experience of the above genetic technologies.

Working independently but in close cooperation and in consultation with the Principal Investigator, the Research Associate II will perform the above laboratory procedures. The incumbent will participate in publications and presentations as author or co-author and they may assist in the preparation of grant proposals but are not responsible for generating grant funds.

ESSENTIAL JOB DUTIES AND RESPONSIBILITIES:

•Perform experiments and will keep appropriate experimental records and documentation and analyze the results with the Principal Investigator.

•Performs the above stated laboratory procedures.

•Operates and maintains equipment and instruments.

•Participation in laboratory meetings, keeping a lab notebook, performing calculations, tabulating data, summarizing methods and results of the related experiments and general lab maintenance will be required.

•Lab cleaning and maintenance duties, including washing and sterilization of glassware, operation of autoclaves and other specialized equipment.

•May be trained on-the-job in basic benchwork under close supervision of PI.

•Performs administrative duties in laboratory or in accordance with research project.

•Maintains project records, as required.

•Orders laboratory supplies, and reagents in a timely manner.

•Assists in maintaining laboratory and laboratory equipment record keeping and genotyping mice strains.

. Harvesting and preparation of mice organs for further analysis such as confocal microscopy based co-immunoanalysis and for single-cell and buld RNA-sequencing.

Educational Requirements:

BS or BA in a biological science or related field.

License/Certification Requirements:

n/a

Experience:

  • Two (2) years of laboratory experience.

  • Understanding of general research objectives.

  • Familiar with routine laboratory procedures, experimental protocols, and overall lab organization.

  • Applicant must be highly motivated and able to work independently.

  • Must have superior interpersonal, communication and organization skills and the ability to work across company disciplines and functional units, and computer application experience.

  • Must possess computer skills to include, but not limited to, Excel, PowerPoint, and Word.

  • Ability to simultaneously manage multiple clerical trials, and attention to details.

Experience with cell culture and animal work is required. Experience with real time PCR, RNA isolation, and cloning including generation of constructs for transfection essential. The applicant should be able to work on weekends and work with mice.

Cedars-Sinai is an EEO employer. Cedars-Sinai does not unlawfully discriminate on the basis of the race, religion, color, national origin, citizenship, ancestry, physical or mental disability, legally protected medical condition (cancer-related or genetic characteristics or any genetic information), marital status, sex, gender, sexual orientation, gender identity, gender expression, pregnancy, age (40 or older), military and/or veteran status or any other basis protected by federal or state law.

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