Cedars-Sinai Research Associate II - Pathology Group Operations - Tourtellotte Lab in Los Angeles, California
The Tourtellotte lab studies the cellular and molecular basis of central and peripheral nervous system development and mechanisms involved in innervation homeostasis and neurodegeneration. We are seeking a Research Technician (Research Associate II) with previous experience in biochemistry, molecular biology, cell biology and/or primary tissue culture who will assist with all aspects of scientific experimentation in the laboratory. The successful candidate will have previous experience working with genetically modified mice, managing a mouse colony of genetically modified mice, and working with mice as experimental models for understanding disease pathogenesis including experiments based on mammalian cell cultures (including induced pluripotent stem cells - iPSCs and iPSC-derived neurons), molecular biology (including construction of DNA vectors, and qPCR) and preparation of samples for sequencing. Under direct supervision, you will perform a variety of routine experiments to support the objectives of one or more laboratory research projects. Assists in general laboratory activities, including maintenance of reagents and stocking of supplies. Keeps accurate and detailed records of experimental procedures and results. Assists in the operation of specialized equipment and instrumentation. Observes safety standards and procedures. You will be able to perform routine cellular and molecular biology procedures including Western blot, Northern blot, Southern blot, DNA/RNA preparation and manipulation, PCR, protein extraction, cell culture, immunohistochemistry, and staining. This position does not have direct supervisory responsibilities. In addition to professional development opportunities, Cedars-Sinai offers competitive compensation and benefits package: Medical, Dental, 403(b) with match in addition to Cedars-Sinai’s paid Retirement Program, Tuition Assistance, Credit Union, Wellbeing Program Rideshare, Employee Discounts, Healthcare and Dependent Care Reimbursement Accounts, Group Life/AD&D and Long Term Disability Insurance, start at 16 vacation days/year.
Demonstrated excellence in planning, executing and analyzing experiments.
Able to troubleshoot, solve complex problems, and develop processes and/or assay improvements.
Attention to detail and careful record-keeping.
Excellent organizational skills and ability to manage multiple projects.
Excellent oral and written communication skills, able to write technical reports and protocols.
Flexible team player motivated to collaborate with internal and external partners.
Essential Job Duties and Responsibilities:
Genotyping genetically modified mice and managing a mouse colony Primary and cell line culture.
Experience Molecular cloning, DNA/RNA purification, PCR/qPCR, DNA/RNA samples for sequencing, immunohistochemistry, and other standard molecular biology techniques Microscopy, including fluorescence in situ hybridization (FISH), immunocytochemistry, tissue immunohistochemistry.
Perform a variety of complex experimental protocols and procedures, and assist in the design of new protocols and techniques.
Keep accurate and detailed records of experiments and results, and assist in identifying and troubleshooting unexpected results.
Order laboratory supplies and support laboratory operations Maintain lab instrumentation and monitor related records and coordinate use of lab equipment.
Orient research laboratory assistants or lower-level research associates and undergraduate/graduate student researchers on day-to-day lab operations.
Position may require some weekend and evening availability as scientific experiments demand
Work independently and collaboratively within 6 months after joining laboratory Have high level of motivation and energy Accept oversight, feedback and mentorship.
Possess high degree of intellectual curiosity, rigor and academic honesty.
Seek continuous improvement and self-education in relevant scientific topics Demonstrate ownership of responsibilities and duties.
Willing to supervise and teach trainees and other research staff
Bachelors Degree is required plus a minumum of 2 years previous experience working in a biomedical research laboratory environment
Masters Degree or Ph.D. plus 3 years of experience in lab working in the area of biochemistry, molecular biology, neuroscience or equivalent is preferred
Demonstrated significant, original, and creative contributions to a research or creative program or project.
Knowledge of laboratory area of research specialization.
Demonstrated technical competency in a variety of research techniques and protocols.
Cedars-Sinai is an EEO employer. Cedars-Sinai does not unlawfully discriminate on the basis of the race, religion, color, national origin, citizenship, ancestry, physical or mental disability, legally protected medical condition (cancer-related or genetic characteristics or any genetic information), marital status, sex, gender, sexual orientation, gender identity, gender expression, pregnancy, age (40 or older), military and/or veteran status or any other basis protected by federal or state law.